In the framework of the European Week of Regions and Cities, CoR organizes this interactive session with the aim to discuss cities’ and regions’ contribution to the formulation and implementation of the post-2020 global and European biodiversity policy framework. Their potential for biodiversity action is not fully utilised at present, although it is increasingly recognised. The ongoing preparatory process for the post-2020 biodiversity agenda represents a major opportunity for local and regional authorities to shape future biodiversity targets and contribute to their delivery, as well as to having their efforts formally recognised by the Member States and the UN Convention on Biological Diversity.